First, the tested hydrogels GrowDex or GrowDex-T were manually diluted with the DMEM cell culture medium and transferred into a glass bottle with a lid. Pressure was added to the bottle using a pressure/vacuum generator (PGVA-1) via a connecting tube. The valve control module VAEM-V precisely controlled the media-separated valve VYKB-F12 in order to dispense the displaced hydrogel mixture into a target vessel. The dispensed quantity was recorded gravimetrically with a pair of scales and used to evaluate the CV value.